Pub. online:1 Jan 2010Type:Research ArticleOpen Access
Volume 21, Issue 3 (2010), pp. 409–424
The paper addresses the over-saturated protein spot detection and extraction problem in two-dimensional electrophoresis gel images. The effective technique for detection and reconstruction of over-saturated protein spots is proposed. The paper presents: an algorithm of the median filter mask adaptation for initial filtering of gel image; the models of over-saturation used for gel image analysis; several models of protein spots used for reconstruction; technique of the automatic over-saturated protein spot search and reconstruction. Experimental investigation confirms that proposed search technique lets to find up to 96% of over-saturated protein spots. Moreover the proposed flexible protein spot shape models for reconstruction are faster and more accurate in comparison to the flexible diffusion model.
Pub. online:1 Jan 2003Type:Research ArticleOpen Access
Volume 14, Issue 4 (2003), pp. 431–444
In this paper, we shall propose a new method for the copyright protection of digital images. To embed the watermark, our new method partitions the original image into blocks and uses the PCA function to project these blocks onto a linear subspace. There is a watermark table, which is computed from projection points, kept in our new method. When extracting a watermark, our method projects the blocks of the modified image by using the PCA function. Both the newly projected points and the watermark table are used to reconstruct the watermark. In our experiments, we have tested our scheme to see how it works on original images modified by JPEG lossy compression, blurring, cropping, rotating and sharpening, and the experimental results show that our method is very robust and indeed workable.